Vector-Alu PCR: a rapid step in mapping cosmids and YACs
نویسندگان
چکیده
منابع مشابه
Vector-Alu PCR: a rapid step in mapping cosmids and YACs.
Economical procedures for mapping large numbers of cosmids and YACs, using panels of somatic cell hybrids are of practical importance in genome mapping. We developed a method based on direct PCR of individual cosmid colonies with primers complementary to the cosmid vector and 2 primers complementary to the extreme 5 prime and 3 prime parts of the Alu consensus sequence (1). We also applied this...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1990
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/18.10.3097